In RNA-seq library preparation, selecting the appropriate number of PCR cycles is a critical balancing act to avoid overcycling.
Conventional PCR systems require users to set a predetermined number of cycles based on assay- and sample-specific factors. When performing single-cell sequencing experiments, this requires knowledge ...
Once the concept of PCR popped into Kary Mullis’ head in 1983 it wasn’t long before scientists realized they had a revolutionary technology in their hands. Because now they could take the smallest ...
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