What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

Structured illumination microscopy (SIM) is the most preferable system for live-cell super-resolution imaging. It enables the observation of intricate subcellular dynamics. However, conventional SIM ...

Top: Random Illumination Microscopy (RIM) utilizes random speckle patterns to achieve super-resolution imaging of biological tissues, improving resolution by a factor of two compared to the optical ...

SIMIP enables high-resolution images rich in both chemical and spatial information. A quantum cascade laser (QCL) excites molecular vibrations while a spatial light modulator (SLM) generates striped ...

For hundreds of years, the optical microscope was the only tool available to scientists wanting to study the movement of cells, bacteria and yeast. But the diffraction of light made it impossible to ...

A project at Swiss research center EPFL has designed an add-on unit intended to bring structured light capability to a standard optical microscope. Described in Nature Communications, the design ...

Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

Nanoscopy is a field of microscopy that focuses on imaging and studying structures and processes at the nanoscale, typically below the diffraction limit of light. It encompasses various techniques ...

Fluorescence microscopy reveals cellular morphology and dynamics in remarkable detail, but achieving clear visualization at fast acquisition rates remains a challenge. A fundamental trade-off between ...

SIM uses multiple images acquired under structured illumination to reconstruct a super-resolved image, exceeding the diffraction limit of traditional microscopy. However, SIM requires acquiring ...